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nudF [2019-11-21 16:38:41]
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nudF [2019-11-21 16:38:41]

involved in isopentenol (isoprenoid) biosynthesis
locus
BSU_23610
pI
4.59
mw
20.84 kDa
protein length
185 aa Sequence Blast
gene length
558 bp Sequence Blast
function
isoprenoid biosynthesis
product
unknown
essential
no
ec
3.6.1.13
synonyms
yqkG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]

    • Gene

    Coordinates
    2,458,509 → 2,459,066

    The protein

    Catalyzed reaction/ biological activity

  • ADP-D-ribose + H2O --> AMP + D-ribose 5-phosphate + 2 H+ (according to UniProt)

    • Protein family

  • [SW|Nudix hydrolase] (according to UniProt)

    • [SW|Domains]

  • [SW|Nudix hydrolase domain] (aa 40-171) (according to UniProt)

    • Structure

  • [PDB|1V8I] (from ''Thermus thermophilus'', 38% identity) [Pubmed|15210687]

    • Expression and Regulation

    Operons

    genes
    [gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]-[gene|9D05B104000EED08FB62D6F362FC5E9C2A5354D9|yqxK]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C410 (yqkG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2408 NBRP B. subtilis, Japan]
  • BKE23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA
  • BKK23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA

    • References

  • 17693564,10542272,15210687,31740579